N.H. Wubshet, A.P. Liu, Methods to mechanically perturb and characterize GUV-based minimal cell models, under review.
Y.-Y. Hsu, A.M. Resto-Irizarry, J. Fu, A.P. Liu, Mechanosensitive channel-based membrane tension biosensor, under revision.
N.H. Wubshet, B. Wu, S. Veerapaneni, A.P. Liu, Differential regulation of GUV mechanics via actin network architectures, Biophysical Journal, accepted, 2022.
S.-S. Lin, J. Joseph, A.P. Liu, Mechanical regulation of exocytosis and endocytosis, Exocytosis from Molecules to Cell, in press, 2022.
Y. Ma, R. Kapoor, B. Sharma, A.P. Liu, A.L. Ferguson, Computational design of self-assembling peptide chassis materials for synthetic cells, Molecular Systems Design & Engineering, in press, 2022.
A. Poddar#, Y.-Y. Hsu#, F. Zhang, C. Muller, M. Malla, A.P. Liu*, Q. Chen*, Membrane stretching activates calcium-permeability of putative fission yeast Pkd2 channel, Molecular Biology of the Cell, in press. # equal contribution, * co-corresponding authors
G. Cai, A. Nguyen, Y. Bashirzadeh, S.-S. Lin, D. Bi, A.P. Liu, Compressive stress drives adhesion-dependent unjamming transitions in breast cancer cell migration, Frontiers in Cell and Developmental Biology, 10:933042, 2022. [article link]
J.G. Joseph, R. Mudgal, S.-S. Lin, A. Ono, A.P. Liu, Biomechanical role of epsin in influenza A virus entry, Membranes, 12(9), 859, 2022. [article link]
S. Majumder, Y.-Y. Hsu#, H. Moghimianavval#, M. Andreas, T.W. Giessen, G.W.G. Luxton, A.P. Liu, In vitro synthesis and reconstitution using mammalian cell-free lysates enables the systematic study of the regulation of LINC complex assembly, Biochemistry, 61, 14, 1495-1507, 2022. # equal contribution
H. Moghimianavval#, C. Patel#, S. Mohapatra#, S.-W. Hwang, T. Kayikcioglu, Y. Bashirzadeh, A.P. Liu*, T. Ha*, Engineering functional membrane-membrane interface by InterSpy, Small, e2202104, 2022. # equal contribution, * co-corresponding authors [article link]
Y. Bashirzadeh, H. Moghimianavval, A.P. Liu, Encapsulated actomyosin patterns drive cell-like membrane shape changes, iScience, 104236, 2022. [article link]
A.P. Liu*, E.A. Appel, P.D. Ashby, B.M. Baker, E. Franco, L. Gu, K.A. Haynes, N.S. Joshi, A.M. Kloxin, P.H.J. Kouwer, J. Mittal, L. Morsut, V. Noireaux, S. Parekh, R. Schulman, S.K.Y. Tang, M.T. Valentine, S.L. Vega, W. Weber, N. Stephanopoulos*, O. Chaudhuri*, The living interface between synthetic biology and biomaterial design, Nature Materials, 21, 390-397, 2022. * co-corresponding authors [article link]
H. Moghimianavval*, Y.-Y. Hsu*, A. Groaz, A.P. Liu, In vitro reconstitution platforms of mammalian cell-free expression of membrane proteins, Methods in Molecular Biology, 2433:105-120, 2022.
M. Luo, G. Cai, K.K.Y. Ho, K. Wen, Z. Tong, L. Deng, A.P. Liu, Compressive stress enhances invasive phenotype of breast cancer cells via Piezo1 activation, BMC Molecular and Cell Biology, 23(1):1, 2022. [article link]
B. Sharma, H. Moghimianavval, S.-W. Hwang, A.P. Liu, Synthetic cell as a platform for understanding membrane-membrane interactions, Membranes, 11, 912, 2021. [article link]
B. Sharma, Y. Ma, H. Hiraki, B.M. Baker, A.L. Ferguson, A.P. Liu, Facile formation of giant elastin-like polypeptide vesicles as synthetic cells, ChemComm, 57, 13202, 2021. [article link]
Y. Bashirzadeh, N.H. Wubshet, T. Litschel, P. Schwille, A.P. Liu, Rapid encapsulation of reconstituted cytoskeleton inside giant unilamellar vesicles, Journal of Visualized Experiments, 177, e63332, 2021. [article link]
N.H. Wubshet, E. Arreguin-Martinez, M. Nail, H. Annamalai, R. Koerner, M. Rousseva, T. Tom, R.B. Gillespie, A.P. Liu, Simulating microgravity using a 3-D clinostat for inducing cellular responses to mechanotransduction in human osteoblasts, Review of Scientific Instruments, 92, 114101, 2021. [article link]
Y.-C Tsan, Y.-T. Zhao, A. Capilnasiu, S.J. DePalma, Y.-W. Wu, B. Elder, I. Panse, S. Friedline, T.S. O'Leary, N. Wubshet, K.K.Y. Ho, M.J. Previs,. B.M, Baker, D. Nordsletten, L.L. Isom, A.P. Liu, A.S. Helms, Biomechanics and myofibrillar alignment drive contractile maturation in stem cell derived cardiac muscle without stromal cells, Nature Communications, 12, 6167, 2021. [article link]
Y. Bashirzadeh, S.A. Redford, C. Lorpaiboon, A. Groaz, T. Litschel, P. Schwille, G.M. Hocky, A.R. Dinner*, A.P. Liu*, Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture, Communications Biology, 4, 2216, 2021. [article link] * co-corresponding authors.
N.H. Wubshet, Y. Bashirzadeh, A.P. Liu, Fascin-induced actin protrusions are suppressed by dendritic networks in GUVs, Molecular Biology of the Cell, 32 (18), 1634-1640, 2021. [article link]
K. Ufford, S. Friedline, Z. Tong, V.T. Tang, A. Dobbs, Y.C. Tsan, S. Bielas, A.P. Liu, A.S. Helms, Myofibrillar developmental variability underlies contractile function in stem cell-derived cardiomyocytes, Stem Cell Reports, 16(3): 470-477, 2021. [article link]
F. Khodadadei, A.P. Liu, C.A. Harris, How is hydrocephalus shunt failure dependent on shear stress? A high-resolution real-time quantification of astrocyte cytokine secretion by shear stress, Communications Biology, 4, 387, 2021. [article link]
B. Sharma, Y. Ma, A.L. Ferguson, A.P. Liu, In search of a novel chassis material for synthetic cells: emergence of synthetic peptide compartment, Soft Matter, 16, 10769-10780, 2020. [article link]
A. Groaz, H. Moghimianavval, F. Tavella, T.W. Giessen, A.G. Vecchiarelli, Q. Yang, A.P. Liu, Engineering spatiotemporal organization and dynamics in synthetic cells, WIREs Nanomedicine & Bionanotechnology, e1685, 2020. [article link]
Y. Bashirzadeh, N.H. Wubshet, A.P. Liu, Confinement geometry tunes fascin-actin bundle structures and consequently the shape of a lipid bilayer vesicle, Frontiers in Molecular Biosciences, 7, 610277, 2020. [article link]
M.S. DeNies, A.V. Smrcka, S. Schnell, A.P. Liu, beta-Arrestin mediates communication between plasma membrane and intracellular GPCRs to regulate signaling, Communications Biology, 3:789, 2020. [article link]
J.G. Joseph, C. Osorio, V. Yee, A. Agrawal, A.P. Liu, Complementary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension, Communications Biology, 3:743, 2020. [article link]
S. Kunisaki, G. Jiang, J. Biancotti, K.K.Y. Ho, B. Dye, A.P. Liu, J. Spence, Human induced pluripotent stem cell-derived lung organoids in an ex vivo model of the congenital diaphragmatic hernia lung, Stem Cells Translational Medicine, 10 (1), 98-114, 2020. [article link]
Y. Liao, J.W. Gose, E.M. Arruda, A.P. Liu, S.D. Merajver, Y.L. Young, The effect of shock impulse on cell viability in vitro, PLoS One, 15(6): e0234138, 2020. [article link]
V. Noireaux, A.P. Liu, The new age of cell-free biology, Annual Review of Biomedical Engineering, 22: 51-77, 2020. [article link]
J.G. Joseph, A.P. Liu, Mechanical regulation of endocytosis: new insights and recent advances, Advanced Biosystems, 1900278, 2020. [article link]
L.K. Rosselli-Murai, J.G. Joseph, I. Lopes-Cendes, A.P. Liu, M.J. Murai, The Machado-Joseph disease-associated form of ataxin-3 impacts dynamics of clathrin-coated pits, Cell Biology International, 44(5), 1252-1259, 2020. [article link]
M.S. DeNies, A.P. Liu, S. Schnell, Are the biomedical sciences ready for synthetic biology? Biomolecular Concepts, 11:23-31, 2020. [article link]
A.S. Helms, V.T. Tang, T.S. O'Leary, S. Friedline, M. Wauchope, A. Arora, A.H. Wasserman, E.D. Smith, L.M. Lee, X. Wen, J.A. Shavit, A.P. Liu, M.J. Previs, S.M. Day, Effects of MYBPC3 loss of function mutations preceding hypertrophic cardiomyopathy, JCI Insight, 5(2):e133782, 2020. [article link]
Y. Bashirzadeh, A.P. Liu, Encapsulation of the cytoskeleton: towards mimicking the mechanics of a cell, Soft Matter, 15, 8425-8436, 2019. [article link]
C. Liu, P. Elvati, S. Majumder, Y. Wang, A.P. Liu, A. Violi, Predicting the time of entry of nanoparticles in lipid membranes, ACS Nano, 13, 10221-10232, 2019. [article link]
J. Loh*, M.-C. Chuang*, S.-S. Lin*, J. Joseph, Y.-A. Su, T.-L. Hsieh, Y.-C. Chang, A.P. Liu, Y.-W. Liu, An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation, Journal of Cell Science, 132, jcs232579, 2019. [article link] * equal contribution.
J. Garamella*, S. Majumder*, A.P. Liu, V. Noireaux, An adaptive synthetic cell based on mechanosensing, biosensing, and inducible gene circuits, ACS Synthetic Biology, 8, 1913-1920, 2019. [article link] * equal contribution, Liu and Noireaux are co-corresponding authors.
S. Majumder, N. Wubshet, A.P. Liu, Encapsulation of complex solutions using droplet microfluidics towards the synthesis of artificial cells, Journal of Micromechanics and Microengineering, 29, 083001, 2019. [article link]
S. Wang, N.J. Emery, A.P. Liu, A novel synthetic toehold switch for microRNA detection in mammalian cells, ACS Synthetic Biology, 8, 1079-1088, 2019. [article link]
A.P. Liu, The rise of bottom-up synthetic biology and cell-free biology, Physical Biology, 16, 040201, 2019. [article link] (Editorial for Special issue on bottom-up synthetic biology)
M.S. DeNies, L.K. Rosselli-Murai, S. Schnell, A.P. Liu, Clathrin heavy chain knockdown influences CXCR4 signaling and post-translational modification, Frontiers in Cell and Developmental Biology, 7, 77, 2019. [article link] (Research Topic on Signaling Compartmentalization Along Endocytic Route)
M. Luo, K.K.Y. Ho, Z. Tong, L. Deng, A.P. Liu, Compressive stress enhances invasive phenotype of breast cancer cells via Piezo1 activation, bioRxiv, 2019. Deng and Liu are co-corresponding authors. [article link]
S. Majumder, P.T. Willey, M. DeNies, A.P. Liu, G.W.G. Luxton, Artificial nuclear membranes: a synthetic biology platform for the reconstitution and mechanistic dissection of LINC complex assembly, Journal of Cell Science, 132, jcs219451, 2019. Liu and Luxton are co-corresponding authors. [article link] (Special issue on Reconstituting Cell Biology)
X. Tan, M. Luo, A.P. Liu, Clathrin-mediated endocytosis regulates fMLP-mediated neutrophil polarization, Heliyon, 4, e00819, 2018. [article link]
K.K.Y. Ho, Y.L. Wang, J. Wu, A.P. Liu, Advanced microfluidic device designed for cyclic compression of single adherent cell, Frontiers of Bioengineering and Biotechnology, 6, 148, 2018. [article link]
M.S. DeNies, L.K. Rosselli-Murai, V.L. Murray, E.M. Steel, A.P. Liu, Proximity biotinylation for studying G protein-coupled receptor dimerization (book chapter). In: Fuxe K., Borroto-Escuela D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY, 2018. [article link]
Z.D. Fox, G. Jiang, K.K.Y. Ho, K. Walker, A.P. Liu, S.M. Kunisaki, Fetal lung transcriptome patterns in an ex vivo compression model of diaphragmatic hernia, Journal of Surgical Research, 231, 411-420, 2018. [article link]
J. Heureaux-Torres, K.E. Luker, H. Haley, M. Pirone, L.M. Lee, Y. Herrera, G.D. Luker, A.P. Liu, The effect of mechanosensitive channel MscL expression in cancer cells on 3D confined migration, APL Bioengineering, 2, 032001, 2018. [article link] Special Topic on Bioengineering of Cancer. Luker and Liu are co-corresponding authors.
S. Wang, S. Majumder, N. Emery, A.P. Liu, Simultaneous monitoring of transcription and translation in mammalian cell-free expression in bulk and in cell-sized droplets, Synthetic Biology, 3(1): ysy005, 2018. [article link]
Y. Guan, Z. Li, S. Wang, P.M. Barnes, X. Liu, H. Xu, M. Jin, A.P. Liu, Q. Yang, A robust and tunable mitotic oscillator in artificial cells, Elife, 7: e33549, 2018. [article link]
L.K. Rosselli-Murai, J.A. Yates, S. Yoshida, J. Bourg, K.K.Y. Ho, M. White, J. Prisby, X. Tan, M. Altemus, L. Bao, Z.-F. Wu, S.L. Veatch, J. Swanson, S.D. Merajver*, A. P. Liu*, Loss of PTEN promotes formation of signaling-specific clathrin-coated pits, Journal of Cell Science, 131, jcs208926, 2018. [article link] Merajver and Liu are co-corresponding authors.
N. Emery, S. Majumder, A.P. Liu, Synergistic nucleic acid production by T7 RNA polymerase and Bsu DNA polymerase in the absence of canonical substrates, Synthetic and Systems Biotechnology, 3, 130-134, 2018. [article link]
F. Ma*, M.T. Chung*, Y. Yao, R. Nidetz, L.M. Lee, A.P. Liu, Y. Feng, K. Kurabayashi, G-Y Yang, Efficient molecular evolution for generating enantioselective enzymes by a dual-channel microfluidic droplet screening platform, Nature Communications, 9:1030, 2018. [article link] Kurabayashi and Yang are co-corresponding authors
S. Majumder, A.P. Liu, Bottom-up synthetic biology: modular design for making artificial platelets, Physical Biology, 15, 023001, 2018. [article link] (Special issue on bottom-up synthetic biology)
X.-S. Wu*, S. Elias*, H. Liu, J.Heureaux, P.J. Wen, A.P. Liu, M.M. Kozlov, L.-G. Wu, Membrane tension inhibits rapid and slow endocytosis in secretory cells, Biophysical Journal, 113, 2406-2414, 2017. [article link] * equal contribution
A.P. Liu*, R.J. Botelho*, C.N. Antonescu*, The big and intricate dreams of little organelles: embracing complexity in the study of membrane traffic, Traffic, 18 (9): 567-579, 2017. [article link] * equal contribution; Botelho and Antonescu are co-corresponding authors
S. Majumder*, J. Garamella*, Y.-L. Wang, M. DeNies, V. Noireaux, A.P. Liu, Cell-sized mechanosensitive and biosensing compartment programmed by DNA, ChemComm, 52, 7349-7352, 2017. [article link] * equal contribution; A.P. Liu and V. Noireaux are co-corresponding authors.
Y. Zheng*, S. Wang*, X. Xue, A. Xu, W. Liao, A. Deng, G. Dai, A.P. Liu, J. Fu, Dll4-Notch signaling in regulating angiogenesis in a 3D biomimetic environment, Lab on a Chip, 17. 1948-1959, 2017. [article link] * equal contribution; A.P. Liu and J. Fu are co-corresponding authors.
A.P. Liu*, O. Chaudhuri*, S. Parekh*, New advances in probing cell-extracellular matrix interactions, Integrative Biology, 9, 383-405, 2017. [article link] * equal contribution and corresponding author (top 10 downloaded article published in Integrative Biology in 2017)
K.K.Y. Ho, J.W. Lee, G. Durand, S. Majumder, A.P. Liu, Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encpasulated mammalian cell-free expression, PLoS One, 12(3): e0174689, 2017. [article link]
E. Irajizad, N. Walani, S.L. Veatch, A.P. Liu, A. Agrawal, Clathrin polymerization exhibits high mechano-geometry sensitivity, Soft Matter, 13, 1455-1462, 2017. [article link]
Q. Zhu, F. Zheng, A.P. Liu, J. Qian, C. Fu, Y. Lin, Shape transformation of the nuclear envelope during closed mitosis, Biophysical Journal, 111, 2309-2316, 2016. [article link]
L.M. Lee*, J.W. Lee*, D. Chase, D. Gebrezgiabhier, A.P. Liu, Development of an advanced microfluidic micropipette aspiration device for single cell mechanics studies, Biomicrofluidics, 10, 054105, 2016. [article link]
K.K.Y. Ho, L.M. Lee, A.P. Liu, Mechanically activated artificial cell by using microfluidics, Scientific Reports, 6, 32912, 2016. [article link]
Z. Peng, O. Pak, A.P. Liu, Y.-N. Young, On the gating of mechanosensitive channels by fluid shear stress, Acta Mechanica Sinica, 32 (6), 1012-1022, 2016. [article link]
P.J. Wen*, S. Grenklo*, E. Hamid, X. Tan, H.S. Liao, J. Heureaux, H.C. Chiang, W. Shin, W.D. Zhao, N. Toilin, E. Evergreen, L. Brodin, R. Karlsson, A. Jin, A.P. Liu, O. Shupliakov, L.G. Wu, Actin and ATP provide mechanical force to collapse fusing vesicles at the plasma membrane, Nature Communications, 7:12604, 2016. [article link]
F. Caschera*, J.W. Lee*, K.K.Y. Ho, A.P. Liu, M.C. Jewett, Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes, ChemComm, 52, 5467-5469, 2016. *equal contribution, M.C. Jewett and A.P. Liu are co-corresponding authors [article link]
A. P. Liu, Biophysical tools for cellular and subcellular mechanical actuation of cell signaling, Biophysical Journal, 111, 1-7, 2016. [article link]
X. Tan, J. Heureaux, A.P. Liu, Cell spreading area regulates clathrin-coated pit dynamics on micropatterned substrate, Integrative Biology, 7, 1033 - 1043, 2015. [article link]
L.M. Lee, A.P. Liu, The application of micropipette aspiration in molecular mechanics of single cells, ASME Journal of Nanotechnology in Engineering and Medicine, 5(4): 040801-04081-6, 2015. (Themed issue on nanotechnology for biology and biomedical applications) [article link]
K.K.Y. Ho*, V.L. Murray*, A.P. Liu, Engineering artificial cells by combining HeLa-based cell free expression and ultrathin double emulsion template, Methods in Cell Biology, volume 128, Chapter 16, 303-318, 2015. *equal contribution [article link]
L.M. Lee, A.P. Liu, A microfluidic pipette array for mechanophenotyping of cancer cells and mechanical gating of mechanosensitive channels, Lab on a Chip, vol 15, 264-273, 2015. [article link]
J. Heureaux, D. Chen, V.L. Murray, C.X. Deng, A.P. Liu, Activation of a bacterial mechanosensitive channel in mammalian cells by cytoskeletal stress, Cellular and Molecular Bioengineering, vol 7 (3), 307-319, 2014. *Young Innovators Special Issue [article link] [journal cover] [featured article]
E.M. Steel*, V.L. Murray*, A.P. Liu, Multiplex detection of homo- and heterodimerization of G protein-coupled receptors by proximity biotinylation, PLoS One, vol 9 (4), e93646, 2014. *equal contribution[article link]
C. Coyne, K. Patel, J. Heureaux, J. Stachowiak, D.A. Fletcher, A.P. Liu, Lipid bilayer vesicle generation using microfluidic jetting. J. Vis. Exp. (84), e51510, doi:10.3791/51510. [article link]
Y. Shao*, X. Tan*, R. Novitski, M. Muqaddam, P.T. List, L. Williamson, J. Fu, A.P. Liu, Uniaxial cell stretching device for high resolution live cell imaging of mechano-sensitive functions, Review of Scientific Instruments, vol 84 114304, 2013. *equal contribution, J. Fu and A.P. Liu are co-corresponding authors [article link]
H. Lin, A.P. Liu, T.H. Smith, J. Trejo, “Dimerization and cofactoring between proteinase-activated receptors: implications for ligand and signaling specificity”, Pharmacological Reviews, vol 65 (4), 1198-213, 2013. [article link]
D. Nunez, C. Antonescu, M. Mettlen, A,P. Liu, S.L. Schmid, D. Loerke, G. Danuser, “Hotspots organize clathrin-mediated endocytosis by efficient recruitment and retention of nucleating resources”, Traffic, vol 12 (12), 1868-78, 2011. [article link]
A.P. Liu, F. Aguet, G. Danuser, S.L. Schmid, “Local clustering of transferrin receptors promotes clathrin-coated pit initiation”, Journal of Cell Biology, vol 191 (7), 1381-1393, 2010. [article link]
D. Banerjee, A.P. Liu, N. Voss, S.L. Schmid, M.G. Finn, “Multivalent display of transferrin on virus-like particles by click chemistry and receptor-mediated endocytosis”, ChemBioChem, vol 11, 1273-1279, 2010.
A.P. Liu, D. Loerke, S.L. Schmid, G. Danuser, “Global and local regulation of clathrin coated pit dynamics detected on patterned substrates”, Biophysical Journal, vol 97 (4), 1038-1047, 2009. [article link]
A.P. Liu, D.A. Fletcher, “Biology under construction: in vitro reconstitution of cellular processes”, Nature Reviews Molecular Cell Biology, vol 10 (9), 644-650, 2009. [article link]
A.P. Liu*, D.L. Richmond*, L. Maibaum, S. Pronk, P.L. Geissler, D.A. Fletcher, “Membrane-induced bundling of actin filaments”, Nature Physics, vol 4, 789-793, 2008. *equal contribution [article link]
J. Stachowiak, D.L. Richmond, T.H. Li, A.P. Liu, S.H. Parekh, D.A. Fletcher, “Giant unilamellar vesicle formation and encapsulation by microfluidic jetting”, Proceeding of National Academy of Sciences, vol 105, 4697-4702, 2008. [article link]
J.L. Choy, S.H. Parekh, O. Chaudhuri, A.P. Liu, C. Bustamante, M.J. Footer, J.A. Theriot, D.A. Fletcher, “Differential force microscope for long-timescale biophysical measurements” Review of Scientific Instruments, vol 78, 043711, 2007. [article link]
A.P. Liu, D.A. Fletcher, “Actin polymerization serves as a membrane domain switch in model lipid bilayer”, Biophysical Journal, vol. 91, 4064-4070, 2006. [article link]
See also New and Notable: M. Edidin, “Switching sides: The actin/membrane lipid connection” Biophysical Journal, vol. 91, 3963, 2006. [article link]
A.P. Liu, D.A. Fletcher, “Photopatterning of actin filament structures”, Nano Letters, vol. 5, no. 4, 625-628, 2005. [article link]
D.H.C. Chen, A.P.C. Liu, Y. Koga, Excess Partial Molar Enthalpy of 1-Propanol in 1-Propanol-Acetone (or Tetramethyl Urea) - H2O at 25oC. Fluid Phase Equil., 189: 31-38, 2001.